No eggs, no larvae does not necessarily mean you are queenless. Here is a simple method to help you know for sure… Shake the bees off of a frame of uncapped brood (eggs and young larvae) from a queen-right colony, being careful not to transfer the queen from that hive. Mark that frame with a magic marker. Remove a frame from the center of the brood chamber of the suspect colony and replace it with your marked frame.
Today’s microsporidian: nosema is frequently misunderstood, even by experienced beekeepers. The confusion appears to stem from failure to recognize the difference between the 2 types of nosema, and the fact that they manifest themselves within the hive in entirely different ways.
Unlike the favorable conditions found in southern latitudes, Pacific Northwest weather has its own set of unique queen-breeding restrictions: Cool, wet, coastal airflow causes unstable maritime conditions that persist well into late spring. The result is both good news and bad news…
Varroa sensitive hygiene (VSH) is a behavioral trait of honey bees (Apis mellifera) in which bees detect and remove bee pupae that are infested by the parasitic mite Varroa destructor. V. destructor is considered to be the most dangerous pest problem for honey bees worldwide. VSH activity results in significant resistance to the mites.
- Harbo, J., and R. Hoopingarner. 1997. Honey Bees (Hymenoptera: Apidae) in the United States that express resistance to Varroa jacobsoni (Mesostigmata: Varroidae). Journal of Economic Entomology 90: 893-898.
- Harbo, J., and J. Harris. 1999. Heritability in honey bees (Hymenoptera: Apidae) of characteristics associated with resistance to Varroa jacobsoni (Mesostigmata: Varroidae). Journal of Economic Entomology 92: 261-265.
- Harbo, J., and J. Harris. 2002. Suppressing Mite Reproduction: SMR an Update. Bee Culture 130: 46-48.
- Harbo, J., and J. Harris. 2005. Suppressed mite reproduction explained by the behavior of adult bees. Journal of Apicultural Research 44: 21-23.
- Ibrahim, A. G. Reuter and M. Spivak. 2006. Field trials of honey bee colonies bred for mechanisms of resistance against Varroa destructor. Apidologie 38: 67-76.
- Harris, J. 2007. 2007. Bees with Varroa Sensitive Hygiene preferentially remove mite infested pupae aged < five days post capping. Journal of Apicultural Research 46: 134-139.
- Ward, K., R. Danka and R. Ward. 2008. Comparative performance of two mite-resistant stocks of honey bees (Hymenoptera: Apidae) in Alabama Beekeeping Operations. Journal of Economic Entomology 101: 654-659.
- Harbo, J., and J. Harris. 2001. Resistance to Varroa destructor (Mesostigmata: Varroidae) when mite-resistant queen honey bees (Hymenoptera: Apidae) were free-mated with unselected drones. Journal of Economic Entomology 94: 1319-1323.
Research Project: Breeding, Genetics, Stock Improvement and Management of Russian Honey Bees for Mite and Small Hive Beetle Control and Pollination
Location: Honey Bee Breeding, Genetics, and Physiology Research
Title: Patriline variation of Nosema ceranae levels in Russian and Italian honey bees
Submitted to: Cold Spring Harbor Meeting
Publication Type: Abstract
Publication Acceptance Date: April 8, 2011
Publication Date: May 8, 2011
Citation: Bourgeois, A.L., Rinderer, T.E., Sylvester, H.A., Holloway, B.A. 2011. Patriline variation of Nosema ceranae levels in Russian and Italian honey bees. Cold Spring Harbor Meeting. 17.
Technical Abstract: The microsporidian Nosema ceranae has invaded managed honey bee colonies throughout the world. While the presence of N. ceranae is common, infection levels are highly variable, even among bees within a single colony. The underlying mechanisms driving this variation are not well-understood. The high degree of individual variation within a colony suggests some degree of genetic resistance to N. ceranae infections may exist among managed honey bee colonies. One likely source for this variation stems from the polygamous nature of honey bee queens, producing multiple patrilines within each colony. We investigated the relationship between infection levels of N. ceranae and patriline membership by sampling individual bees from colonies from both Russian and Italian stocks. A total of 720 bees were collected from 5 Russian and 5 Italian colonies. Individual bees were tested for N. ceranae infection levels using qPCR, and were genotyped to determine patriline membership. Levels of N. ceranae varied significantly at the stock level (Russian: 3.68 x 106 ± 1.88 x 106 nosema/bee and Italian: 9.14 x 106 ± 4.62 x 106 nosema/bee; P = 0.008) and at the colony level for both Russian (P = 0.002) and Italian (P = 0.003) bees. Patriline-based variance was evident among only the Russian bees (P = 0.024). There was substantial variation in N. ceranae levels among Italian bees, ranging from 0 to 2.12 x 109 nosema/bee, however this variation was not associated with patriline membership (P = 0.742). The variance in N. ceranae infection among Russian honey bee patrilines demonstrates a genetic basis for resistance to N. ceranae infection which conforms to predictions of models that relate patriline variance and abundance to disease resistance in honey bees. This difference between Russian and Italian honey bees may derive from Italian honey bees having only a short history of exposure to N. ceranae while Russian honey bees may have had 150 years of exposure.
Last Modified: 02/05/2012
The finding, published recently in the Journal of Insect Physiology, suggests that giving honey bees access to a greater quantity and variety of pollen—their only source of protein—could make them more resilient against parasites and other pests, and help to stem worrisome declines in bee populations.
History of OWA Russian Honeybees
Sample#1 (FERAL BEES)
This is our strongest breeder colony. She is a 2009 Queen. Entirely untreated; has never exhibited symptoms of dwindling; and has had 4 to 5 brood combs removed and added to weaker colonies throughout the 2011 season, yet remains vigorous.
This colony has been tested in the USDA Beltsville Lab 4 times with these results:
- March 2010 = 21.55 Nosema spores/bee (million)
- May 2010 = 10.45 Nosema spores/bee (million)
- June 2010 = 4.40 Nosema spores/bee (million)
- Feb 2011 = 1.30 Nosema spores/bee (million)
Sample was also submitted to Dave Wick at BVS, Inc. Testing indicatd the presence of IAPV, CPV and Sac Brood virus. (Dave did confirm that IAPV and black queen cell virus are associated with n.ceranae, however he was not able to confirm that CPV is).
Only 5% of our 2011 colonies showed no sign of dwindling after their 4th cool; wet; protracted winter.
Now in the 5th spring since the devastating losses from this pathogen, our untreated bees are thriving in spite of staggering nosema spore loads and associated viruses, and we have had only 10% winter loss in 2011-2012.